{"created":"2023-06-20T14:34:47.888793+00:00","id":174,"links":{},"metadata":{"_buckets":{"deposit":"e294bb2c-21ed-4502-92d3-adf701dff584"},"_deposit":{"created_by":3,"id":"174","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"174"},"status":"published"},"_oai":{"id":"oai:kjunshin.repo.nii.ac.jp:00000174","sets":["1:38"]},"author_link":["550","558","551","560","554","555","549","559","552","556","553","557"],"item_1_biblio_info_14":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"9","bibliographicPageStart":"1","bibliographicVolumeNumber":"11","bibliographic_titles":[{"bibliographic_title":"鹿児島純心女子大学看護栄養学部紀要"},{"bibliographic_title":"Bulletin of Faculty of Nursing and Nutrition Kagoshima Immaculate Heart University","bibliographic_titleLang":"en"}]}]},"item_1_creator_6":{"attribute_name":"著者名(日)","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"海老原, 綾子","creatorNameLang":"ja"},{"creatorName":"エビハラ, アヤコ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"海老原","familyNameLang":"ja"},{"familyName":"エビハラ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"綾子","givenNameLang":"ja"},{"givenName":"アヤコ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"549","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"中, 佳子"}],"nameIdentifiers":[{"nameIdentifier":"550","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"西田, 友佳"}],"nameIdentifiers":[{"nameIdentifier":"551","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"大久保, 美智子"}],"nameIdentifiers":[{"nameIdentifier":"552","nameIdentifierScheme":"WEKO"}]}]},"item_1_creator_7":{"attribute_name":"著者名よみ","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"エビハラ, アヤコ"}],"nameIdentifiers":[{"nameIdentifier":"553","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"アタリ, ヨシコ"}],"nameIdentifiers":[{"nameIdentifier":"554","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"ニシダ, ユカ"}],"nameIdentifiers":[{"nameIdentifier":"555","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"オオクボ, ミチコ"}],"nameIdentifiers":[{"nameIdentifier":"556","nameIdentifierScheme":"WEKO"}]}]},"item_1_creator_8":{"attribute_name":"著者名(英)","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ebihara, Ayako","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"557","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Atari, Yoshiko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"558","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nishida, Yuka","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"559","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ohkubo, Michiko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"560","nameIdentifierScheme":"WEKO"}]}]},"item_1_description_1":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_type":"Other"}]},"item_1_description_11":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_description":"プロテアーゼインヒビターは,タンパク質分解作用に関わるのみでなく,さまざまな生理作用を持ち,プロテアーゼの制御因子として重要な役割を果たしている。モロヘイヤから70%硫安分画法,ラウリル硫酸ナトリウムポリアクリルアミドゲル電気泳動(SDS-PAGE)法を用いて,トリプシンインヒビター(TI)活性を持ったタンパク質を分離精製した。モロヘイヤTI活性の測定にあたっては,モロヘイヤ70%硫安画分,トリプシン,基質としてカゼインを混和し,37℃,20分インキュベート後,トリクロロ酢酸を加え,遠心分離し,その上清の280nmの吸光度を分光光度計で測定した。その結果,TI存在下の反応液の吸光度の増加はみられなかった。プロテアーゼインヒビターのタンパク質をSDS-PAGE法を用いて解析した結果,100, 59, 55, 50kDaの分子量のタンパク質が存在することが分かった。非還元条件下でのSDS-PAGE解析の結果では,モロヘイヤのTIタンパク質の分子量は, 100kDa であることが分かった。TI活性の熱に対する安定性について,SDS-PAGE法を用いて解析したところ, 100℃で10分間処理したTIタンパク質は,トリプシンを阻害することはできなかった。","subitem_description_type":"Other"}]},"item_1_description_12":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"The protease inhibitor may be playing an important role in the metabolism of the plant restraining the endogenous proteinases or protecting the plant against foreign proteinases. A trypsin inhibitor(TI) was isolated and purified from the tossa jute (Corchorus olitorius L.) juice by 70% ammonium sulfate treatment. The inhibitory activity of TI was examined by photometical method and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The trypsin activity was analyzed by incubating 70% ammounium sulfate fraction of tossa jute mixed with trypsin and casein as substrate. The mixture was incubated at 37℃ for 20 minutes before trichloroacetic acid (TCA) solution was added to stop the process. After centrifugation, the absorbance of the supernatant of this reaction mixture was measured at 280nm with a spectrophotometer. The absorbance at 280nm of the reaction mixture containing TI has not increased at all. TI inhibited trypsin activities which catalyzed casein. Analyzed by SDS-PAGE, the molecular weights of TI was found to be 100, 59, 55, 50kDa. Under non-reducing conditions in SDS-PAGE gel, TI showed a single protein band with estimated molecular weight of 100kDa. After being treated at 100℃ for 10 minutes, TI could no longer inhibit trypsin activities, which proved that the trypsin-inhibitory activity of TI was heat-unstable.","subitem_description_type":"Other"}]},"item_1_source_id_13":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AA11824432","subitem_source_identifier_type":"NCID"}]},"item_1_text_10":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Department of Nutrition, Faculty of Nursing and Nutrition, Kagoshima Immaculate Heart University"},{"subitem_text_language":"en","subitem_text_value":"Department of Nutrition, Faculty of Nursing and Nutrition, Kagoshima Immaculate Heart University"},{"subitem_text_language":"en","subitem_text_value":"Department of Nutrition, Faculty of Nursing and Nutrition, Kagoshima Immaculate Heart University"},{"subitem_text_language":"en","subitem_text_value":"Department of Nutrition, Faculty of Nursing and Nutrition, Kagoshima Immaculate Heart University"}]},"item_1_text_9":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_value":"鹿児島純心女子大学看護栄養学部健康栄養学科"},{"subitem_text_value":"鹿児島純心女子大学看護栄養学部健康栄養学科"},{"subitem_text_value":"鹿児島純心女子大学看護栄養学部健康栄養学科"},{"subitem_text_value":"鹿児島純心女子大学看護栄養学部健康栄養学科"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2007-01-01"}],"displaytype":"detail","filename":"EBIHARA-KA11.pdf","filesize":[{"value":"662.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"url":"https://kjunshin.repo.nii.ac.jp/record/174/files/EBIHARA-KA11.pdf"},"version_id":"289e316b-7296-464f-a5bc-c477221ea7d7"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"モロヘイヤ","subitem_subject_scheme":"Other"},{"subitem_subject":"硫酸アンモニウム分画","subitem_subject_scheme":"Other"},{"subitem_subject":"トリプシンインヒビター","subitem_subject_scheme":"Other"},{"subitem_subject":"SDS-PAGE法","subitem_subject_scheme":"Other"},{"subitem_subject":"tossa jute","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"ammonium sulfate treatment","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"trypsin inhibitor","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"SDS-PAGE","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"モロヘイヤ中のトリプシンインヒビターについて","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"モロヘイヤ中のトリプシンインヒビターについて"},{"subitem_title":"Study on Trypsin Inhibitor from Tossa Jute","subitem_title_language":"en"}]},"item_type_id":"1","owner":"3","path":["38"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-01-01"},"publish_date":"2007-01-01","publish_status":"0","recid":"174","relation_version_is_last":true,"title":["モロヘイヤ中のトリプシンインヒビターについて"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-12-16T01:57:13.288136+00:00"}